|
週次 |
日期 |
單元主題 |
|
第1週 |
2/19 |
課程介紹與分組，實驗室安全守則，值日生分配
|
|
第2週 |
2/26 |
國定假日 |
|
第3週 |
3/05 |
Basic Laboratory Techniques
Experiment 1: Culture transfer techniques
Experiment 2: Techniques for isolation of pure cultures
Experiment 3: Cultural characteristics of microorganisms
|
|
第4週 |
3/12 |
Microscopy
Experiment 4: Microscopic examination of stained cell preparations
Experiment 5: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount
|
|
第5週 |
3/19 |
Bacterial Staining-I
Experiment 6: Preparation of bacterial smears
Experiment 7: Simple staining
Experiment 8: Negative staining
Experiment 9: Gram stain
|
|
第6週 |
3/26 |
Bacterial Staining-II
Experiment 10: Acid-fast stain
Experiment 11: Differential staining for visualization of bacterial cell structure
Experiment: Flagella stain
|
|
第7週 |
4/02 |
清明連假 |
|
第8週 |
4/09 |
Experiment 13: Use of differential and selective media
Experiment 14: Physical factors: temperature
Experiment 15: Physical factors: pH of the extracellular environment
Experiment 16: Physical factors: atmosphere oxygen
Requirements
|
|
第9週 |
4/16 |
期中考週停課 |
|
第10週 |
4/23 |
Experiment 18: Serial dilution-agar plate procedure to
quantitate viable cells
Experiment 19: The bacterial growth curve
Bacterial Genetics-I
Experiment 54: Isolation of a streptomycin-resistant
mutant
Experiment 56: Bacterial transformation |
|
第11週 |
4/30 |
Bacterial Genetics-II
Experiment: Bacterial conjugation and transposition
Experiment 52: Enzyme induction
Experiment 55: The Ames test: A bacterial test system for chemical carcinogenicity
|
|
第12週 |
5/07 |
Biochemical Activities of Microorganisms-I
Experiment 20: Extracellular enzymatic activities of microorganism
Experiment 21: Carbohydrate fermentation
Experiment 22: Triple sugar-iron agar test
Experiment 23: IMViC test
Experiment 24: Hydrogen sulfide test |
|
第13週 |
5/14 |
Biochemical Activities of Microorganisms-II
Experiment 25: Urease test
Experiment 26: Litmus milk reactions
Experiment 27: Nitrate reduction test
Experiment 28: Catalase test
Experiment 29: Oxidase test
|
|
第14週 |
5/21 |
Experiment 37: Cultivation and enumeration of bacteriophages
Experiment 47: Standard qualitative analysis of water
|
|
第15週 |
5/28 |
Physical and Chemical Agents for the Control of Microbial Growth-I
Experiment 40: Physical agents of control-moist heat
Experiment 41: Physical agents of control-electromagnetic radiations
Experiment 42: Chemical agents of control-chemotherapeutic agents
|
|
第16週 |
6/04 |
Physical and Chemical Agents for the Control of Microbial Growth-II
Experiment 43: Determination of penicillin activity in the presence and absence of penicillinase
Experiment 44: Chemical agents of control-disinfectants and antiseptics |
|
第17週 |
6/11 |
期末考
Experiment 31: Genus identification of unknown bacterial cultures
綜合討論，教學意見調查， 實驗室整理 |